In the brightfield mode, the light is transmitted from below the sample that shows up vividly showing the form and structure of the specimen on a bright background. With darkfield illumination, the transmitted light will not reach the microscope and will therefore not be part of the image.
The image will show bright objects on an almost black background. This type of light is not only suitable for prepared specimen but also for live specimen such as flagellum, the pseudopod movement, spirochete and more. Both types of illumination allow for high contrast, clear images and adds a whole new dimension to the Dino-Lite digital handheld microscopes with magnifications over 100x.